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Anaerobic bacteria can be identified by growing them in test tubes of thioglycollate broth:
1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
2: Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
3: Facultative anaerobes can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration.
4: Microaerophiles need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube but not the very top.
5: Aerotolerant organisms do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes however, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube.

산소내성 혐기성 미생물(aerotolerant anaerobe)은 무산소적으로 에너지 대사를 하기 때문에 산소가 필요없는 미생물이다. 이 미생물은 절대혐기성 미생물과 달리 산소의 독성을 제거할 수 있는 효소가 있다. 따라서 이 미생물은 배양 튜브의 전체에 골고루 퍼져서 자란다.


산소내성 혐기성 미생물은 초과산화물 불균등화효소(superoxide dismutase)와 과산화효소(peroxidase)는 있지만 카탈레이스(catalase)는 없다.[1]

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